![]() ![]() The macroscopic lesioned area after cold and hot compresses. The interquartile range is shownas a box with the median marked as a horizontal line minimum and maximum from lower and upper quartile represent error bar. Box plot explanation: The median for each group is shown as a box plot with whiskers from minimum to maximum. Both cold and hot compresses were applied 20 min or 3 hrs four times every 6 hrs. Consequently, these results suggested that the cold pack for the extravasation of DOX might reduce inflammation.Ĭ5a receptor (CD88) Hot and cold compresses doxorubicin interleukin-8 receptor transient receptor potential cation channel subfamily V member 1.Įffects of compress application on the size of lesion area, neutrophilic infiltrate, and vascular permeability. mRNA for CD88, IL-8RA and TRPV1 was detectable by reverse transcription-polymerase chain reaction in both the cold and hot pack groups. The number of nerve fascicles expressing TRPV1 was higher in the hot group than in the cold group on days 1, 3 and 14. The numbers of inflammatory cells expressing CD88 and IL-8RA were significantly lower in the cold group than that in the other groups at almost time points and in the hot groups at later time points, respectively. The neutrophil count in the lesion was significantly higher in the hot group than in the cold (3 hrs) and control groups. Macroscopic observation showed that the area of the skin lesion was significantly smaller in the cold group than in the control group, but was significantly larger in the hot group. Immunofluorescence and RT-PCR for C5a receptor (CD88), interleukin-8 receptor (IL-8RA), and transient receptor potential cation channel subfamily V member 1 (TRPV1) were also performed. We injected 20 μl of doxorubicin (DOX) (1 μg/μl) subcutaneously into the dorsal area in mice and observed the resulting skin lesions macroscopically and histologically from day 1 to day 14 thereafter in groups treated with a cold pack (18-20☌) and a hot pack (38-40☌) or left untreated (control). In the present study, we investigated a part of the mechanism responsible for the effects of hot and cold compresses for extravasation of doxorubicin.
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